Using hydroxyl radical footprinting to explore the free energy landscape of protein folding
نویسندگان
چکیده
منابع مشابه
Using hydroxyl radical footprinting to explore the free energy landscape of protein folding
Characterisation of the conformational states adopted during protein folding, including globally unfolded/disordered structures and partially folded intermediate species, is vital to gain fundamental insights into how a protein folds. In this work we employ fast photochemical oxidation of proteins (FPOP) to map the structural changes that occur in the folding of the four-helical bacterial immun...
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The NK fitness landscape is a mathematical landscape model with a parameter k that governs the degree of ruggedness of the landscape. We presented a procedure to fit a given landscape to the NK fitness landscape by introducing the "apparent k-value"k(app). In this paper, we defined the protein free energy (DeltaG) landscape in amino acid sequence space, where DeltaG is the folding free energy f...
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ژورنال
عنوان ژورنال: Methods
سال: 2015
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2015.02.018